Bioinformatic screening and immunoinfiltration analysis for novel genes of colorectal cancer liver metastasis
[Objective]To screen new key genes and provide potential therapeutic target for colorectal cancer liver metastasis based on bioinformatics technology.[Method]Three datasets,GSE221240,GSE41258 and GSE6988,were obtained from the GEO database.Differential genes(DEGs)were screened for GO enrichment analysis and KEGG pathway analysis.Protein in-teraction network(PPI)was constructed.GEPIA,HPA,TIMER 2.0 databases were used for differential expression analysis,survival analysis,immunohistochemical staining level and immune infiltration analysis to verify the reliability of key genes.[Result]A total of 30 DEGs were obtained,with 28 up-regulated genes and 2 down-regulated genes in liver metastasis sam-ples.GO enrichment and KEGG pathway analysis were mainly involved in acute phase reaction,lipid hydroxylation,DNA ad-duction chemical carcinogenesis,cytochrome P450 drug metabolism and other processes.PPI contained 28 protein nodes and 175 edges.Differential expression analysis and survival analysis verified two novel colorectal cancer liver metastasis genes named CYP2E1 and ALDOB(P＜0.05).Immunohistochemical staining showed that CYP2E1 and ALDOB were significantly different in normal colorectal tissues,colorectal cancer tissues and liver tissues.The results of immunoinfiltration illustrated that the expression levels of CYP2E1 and ALDOB were significantly positively correlated with various immune cells(P＜0.05).[Conclusion]Two new key genes of colorectal cancer liver metastasis,CYP2E1 and ALDOB,were identified by bioinformatic screening.The expression of CYP2E1 and ALDOB in liver tissues is significantly upregulated compared with that in colorectal tissues.Besides,the level of CYP2E1 and ALDOB expression is significantly correlated with a variety of immune cell infiltra-tion.To sum up,CYP2E1 and ALDOB is expected to become new targets for prognosis evaluation and treatment of colorectal cancer liver metastasis.
万方数据
维普
