摘要
[目的]获得猪METTL3蛋白理化性质、二、三级结构、蛋白保守性、甲基化位点及靶基因等信息,为进一步了解猪METTL3基因的功能提供了理论依据.[方法]利用生物信息学软件对猪METTL3蛋白质的理化性质、二、三级结构及保守结构域、甲基化位点、蛋白互作和靶基因等进行预测分析.[结果]猪METTL3为亲水性不稳定蛋白,主要由无规卷曲和α-螺旋、β-转角构成,主要存在于细胞核中,与METTL14、WTAP和POLR2B等蛋白具有互作关系.METTL3有 4 个高可信度 m6A 位点,经 M6A2 Target 软件预测该基因与 CASP1、PYCR1、RANBP2、LIRB4、APOBEC3A、ADAM19 等134个基因存在靶向关系.[结论]猪METTL3是一种主要存在于细胞核中的亲水性不稳定蛋白,主要通过m6A甲基化修饰作用于靶基因而发挥作用.
Abstract
[Objective]The physical and chemical properties,secondary and tertiary structure,protein conservation,methyla-tion site and target gene of porcine METTL3 protein were obtained,which provided theoretical basis for further understanding the function of porcine METTL3 gene.[Method]The physical and chemical properties,secondary and tertiary structures,con-served domains,methylation sites,protein interactions and target genes of porcine METTL3 protein were predicted by bioinfor-matics software.[Result]Porine METTL3 is a hydrophilic unstable protein,mainly composed of random curling,α-helix andβ-corner,which mainly exists in the nucleus and has interaction with METTL14,WTAP and POLR2B proteins.METTL3 has four high-confidence m6A sites,and M6A2 Target software predicted that this gene has a targeting relationship with 134 genes,including CASP1,PYCRI,RANBP2,LIRB4,APOBEC3A and ADAM 19.[Conclusion]Porcine METTL3 is a hydrophilic unstable protein that exists mainly in the nucleus and exerts its effects on target genes mainly through m6A methylation modification.
基金项目
甘肃省教育科技创新项目(2024A-162)
陇东学院创新创业启动基金项目(202301144)
陇东学院博士科研启动基金项目(XY-BYZK2210)
维普
万方数据