[目的]探讨miR-346靶向基质金属蛋白酶-9(MMP-9)对绒毛膜癌细胞株JAR细胞侵袭增殖的影响。[方法]设绒毛膜癌细胞株JAR组、miR-NC组、miR-346 mimics组、miR-346 inhibitor组，培养72 h。MTT法检测绒毛膜癌细胞株JAR活力、癌细胞细胞集落，流式细胞仪检测细胞凋亡水平，Transwell实验测定细胞侵袭水平，RT-PCR法及蛋白电泳法测定miR-346、MMP-9水平。[结果]与绒毛膜癌细胞株JAR组和miR-NC组比较，miR-346 mimics组OD值、存活率、细胞集落、穿膜数、迁移距离、MMP-9 mRNA和蛋白水平降低[0。73±0。03、0。72±0。03 vs 0。41±0。04，(78。24±6。25、76。37±7。35 vs 42。74±7。34)％，619。47±89。35、614。88±75。27 vs 239。32±35。53，693。85±184。74、702。28±111。52 vs 293。85±95。28，(100。25±18。55、98。54±17。45 vs 68。25±12。24)µm，3。85±0。25、3。90±0。23 vs2。04±0。24，0。90±0。22、0。89±0。23 vs 0。37±0。26]，细胞凋亡率、miR-346 水平[(12。38±1。37、12。26±1。20 vs 25。39±1。47)％，3。17±0。33、3。15±0。36 vs 5。68±0。37]升高(P＜0。05)，miR-346 inhibitor 组 OD 值、存活率、细胞集落、穿膜数、迁移距离、MMP-9 mRNA 和蛋白水平(0。90±0。04，86。95％±6。24％，1062。28±102。79，1917。34±425。35，198。25 μm±25。66 μm，4。63±0。28，1。48±0。34)升高，细胞凋亡率、miR-346 水平(6。62±0。54，1。42±0。38)降低(P＜0。05)。[结论]miR-346对绒毛膜癌细胞株JAR细胞侵袭、增殖具有明显抑制作用，其机制可能与miR-346靶向抑制MMP-9表达有关。
Inhibition of miR-346 targeting MMP-9 on invasion and proliferation of JAR cells in chorionic cancer cell lines
[Objective]To explore the effect of miR-346 targeting matrix metalloproteinase-9(MMP-9)on invasion and proliferation of JAR cells in chorionic cancer cell lines.[Method]Chorionic cancer cell lines JAR group,miR-NC group,miR-346 mimics group and miR-346 inhibitor group were cultured for 72 hours.JAR activity and cell colony of chorionic cancer cell lines were detected by MTT assay,apoptosis level was detected by flow cytometry,cell invasion level was determined by Tr-answell assay,and miR-346 and MMP-9 levels were determined by RT-PCR and protein electrophoresis.[Result]Com-pared with the choriocarcinoma cell line JAR group and the miR-NC group,the miR-346 mimics group showed decreased OD values,survival rate,cell colonies,membrane penetration number,migration distance,MMP-9 mRNA and protein levels[0.73±0.03,0.72±0.03 vs 0.41±0.04;(78.24±6.25,76.37±7.35 vs 42.74±7.34)％;619.47±89.35,614.88±75.27 vs 239.32±35.53;693.85±184.74,702.28±111.52 vs 293.85±95.28;(100.25±18.55,98.54±17.45 vs 68.25±12.24)µm;3.85±0.25,3.90±0.23 vs 2.04±0.24;0.90±0.22,0.89±0.23 vs 0.37±0.26].And the apoptosis rate,levels of miR-346[(12.38±1.37,12.26±1.20 vs 25.39±1.47)％;3.17±0.33,3.15±0.36 vs 5.68±0.37)increased(P＜0.05),and the OD value,survival rate,cell colony,membrane penetration number,migration distance,MMP-9 mRNA and protein levels(0.90±0.04,86.95％±6.24％,1062.28±102.79,1917.34±425.35,198.25 µm±25.66 μm,4.63±0.28,1.48±0.34)increased in the miR-346 inhibitor group,while the apoptosis rate and miR-346 level(6.62±0.54,1.42±0.38)decreased(P＜0.05).[Conclusion]miR-346 can significantly inhibit the invasion and proliferation of JAR cells in chorionic cancer cells,and the mechanism may be related to the targeted inhibition of MMP-9 expression by miR-346.
万方数据
维普
