首页|PSMC4表达下调促进MEF细胞衰老

PSMC4表达下调促进MEF细胞衰老

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[目的]探讨PSMC4表达下调对小鼠胚胎成纤维(Mouse Embryonic Fibroblast，MEF)细胞衰老的影响。[方法]利用小干扰RNA(siRNA)敲低PSMC4，荧光定量PCR和Western Blotting验证siRNA的敲低效果;通过β-半乳糖苷酶染色检测细胞衰老情况;通过EdU检测细胞增殖;通过酶联免疫吸附法检测26S蛋白酶体活性。[结果]下调PSMC4后β-半乳糖苷酶染色阳性细胞数量明显增加，EdU细胞增殖率降低约2。7倍(P＜0。01)，26S蛋白酶体活性降低约1。6倍(P＜0。01)。[结论]PSMC4表达下调通过降低26S蛋白酶体活性抑制MEF细胞增殖，进而促进MEF细胞衰老。
Down-regulation of PSMC4 expression promotes MEF cell senescence
[Objective]To investigate the impact of down-regulated PSMC4 expression on Mouse Embryonic Fibroblast(MEF)cell senescence.[Method]PSMC4 was targeted for knockdown using small interfering RNA(siRNA),and the knock-down efficiency was validated by quantitative PCR and Western Blotting.Cellular senescence was detected by β-galactosi-dase.Cellular proliferation was evaluated using EdU.26S proteasome activity was determined via enzyme-linked immunosor-bent assay.[Result]Down-regulation of PSMC4 significantly increased the number of β-galactosidase staining positive cells,decreased the proliferation rate of EdU cells by about 2.7-fold(P＜0.01),and decreased the 26S proteasome activity by a-bout 1.6-fold(P＜0.01).[Conclusion]The down-regulation of PSMC4 expression hindered MEF cell proliferation by di-minishing 26S proteasome activity,consequently promoting MEF cell senescence.

proteasomemouse embryonic fibroblastsPSMC4siRNAcell senescencecell proliferation

骆诗凌、谢健鸿、吴世裕、曾芷彤、刘新光、袁源

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广东省医学分子诊断重点实验室,广东东莞 523808

广东医科大学衰老研究所,广东东莞 523808

广东医科大学生物化学与分子生物学研究所,广东东莞 523808

蛋白酶体 小鼠胚胎成纤维细胞 PSMC4 siRNA 细胞衰老 细胞增殖

国家自然科学基金面上项目国家自然科学基金面上项目广东医科大学博士学位人员科研启动基金项目广东省大学生创新创业训练计划项目广东医科大学大学生创新实验项目广东医科大学大学生创新实验项目

8197130981671399GDMUB2023005S2022105710662020ZYDB0022020ZZDB003

2024

10.16519/j.cnki.1004-311x.2024.05.0079

生物技术
黑龙江省微生物学会 黑龙江省生物工程学会 黑龙江省科学院微生物研究所

生物技术

CSTPCD
影响因子：0.611
ISSN：1004-311X
年,卷(期)：2024.34(5)