[目的]探讨miR-143-3p与HNRNPC在鼻咽癌细胞中的相互作用关系。[方法]将鼻咽癌细胞分为4组:miR NC组、miR-143-3p mimic组、pcDNA3。1 NC组和pcDNA3。1 HNRNPC组。用结晶紫染色检测单克隆形成数目、流式细胞术检测细胞凋亡情况，同时RT-PCR和免疫组化实验测定miR-143-3p、HNRNPC的表达水平，蛋白免疫印迹法测定HNRNPC蛋白的表达水平。用生信学方法预测miR-143-3p与HNRNPC的结合位点，荧光素酶报告基因检测miR-143-3p对CNE2细胞HNRNPC活性的调控作用。[结果]miR-143-3p在鼻咽癌组织中的表达水平显著降低(1。25±0。03 vs 3。67±0。06)，然而HNRNPC在鼻咽癌组织的表达水平升高(1。32±0。05 vs 0。46±0。03)。此外，miR-143-3p能够靶向抑制HNRNPC表达。在鼻咽癌细胞系中，miR-143-3p mimic处理完细胞之后，鼻咽癌细胞增殖能力受到抑制(58。15±7。28 vs 13。27±3。56)，凋亡增加(9。05±0。28 vs 30。07±1。59)％。pcDNA3。1 HNRNPC处理完细胞之后，鼻咽癌细胞增殖能力提高(61。22±5。38 vs 113。57±5。87)，凋亡减少(9。33±0。08 vs 5。01±0。09)％。[结论]miR-143-3p在鼻咽癌组织中低表达，miR-143-3p通过靶向作用HNRNPC进而抑制鼻咽癌细胞的生长，并促进鼻咽癌细胞凋亡。
miR-143-3 p inhibits in vitro metastasis activity of nasopharyngeal carcinoma by targeting and regulating HNRNPC
[Objective]To investigate the interaction between miR-143-3p and HNRNPC in nasopharyngeal carcinoma cells.[Method]NPC cells were divided into four groups:miR NC group,miR-143-3p mimic group,pcDNA3.1 NC group and pcDNA3.1 HNRNPC group.The number of monoclonal colonies was detected by crystal violet staining,and cell apoptosis was measured by flow cytometry.The level of miR-143-3p and HNRNPC were determined by RT-PCR and immunohistochemis-try,and the expression levels of HNRNPC proteins were determined by Western Blot.The binding sites of miR-143-3p and HNRNPC were predicted by bioinformatics methods,and the regulation effect of miR-143-3p on HNRNPC activity in CNE2 cells was detected by luciferase reporter gene.[Result]The expression level of miR-143-3p was significantly decreased in NPC tissues(1.25±0.03 vs 3.67±0.06),while the expression level of HNRNPC was increased in NPC tissues(1.32±0.05 vs 0.46±0.03).In addition,miR-143-3p could target and inhibit HNRNPC expression.After treatment with miR-143-3p mimic,the proliferation ability of NPC cells was inhibited(58.15±7.28 vs 13.27±3.56),and the apoptosis rate was in-creased(9.05±0.28 vs 30.07±1.59)％.After treatment with pcDNA 3.1 HNRNPC,the proliferation ability of NPC cells was increased(61.22±5.38 vs 113.57±5.87),and the apoptosis was decreased(9.33±0.08 vs 5.01±0.09)％.[Conclusion]miR-143-3p is lowly expressed in nasopharyngeal carcinoma tissues.miR-143-3p inhibits the growth of nasopharyngeal carcinoma cells and promotes the apoptosis of nasopharyngeal carcinoma cells by targeting HNRNPC.
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