[目的]探究SHP-1与NF-κB对深静脉血栓血清炎症因子水平的影响。[方法]通过免疫组化和蛋白免疫印迹实验分析下肢静脉血管组织中的SHP-1表达水平;将深静脉血栓患者的外周血单个核细胞分为3组:对照组，pcD-NA NC组和pcDNA SHP-1组。通过酶联免疫吸附试验分析外周血单个核细胞分泌的IL-1β、IL-6、IFN-γ和TNF-α的浓度;通过蛋白免疫印迹方法检测不同实验组的外周血单个核细胞中SHP-1以及NF-κB p65的蛋白水平。[结果]免疫组化实验结果显示，SHP-1在深静脉血栓患者的血管组织中表达降低(P＜0。05)，NF-κB p65在深静脉血栓患者的血管组织中血管组织中表达增加(0。21±0。01 vs0。82±0。05，P＜0。05);酶联免疫吸附试验结果显示，pcDNA SHP-1组的炎症因子IL-1 β、IL-6、IFN-γ和TNF-α的释放水平下降(P＜0。05);蛋白免疫印迹实验结果显示，pcDNA SHP-1 组的 SHP-1 蛋白表达水平增加(0。19±0。02 vs 0。22±0。03 vs 0。82±0。06，P＜0。05)，而 NF-κBp65蛋白表达水平降低(0。71±0。03 vs 0。73±0。05 vs 0。25±0。02，P＜0。05)。[结论]在深静脉血栓患者体内，SHP-1表达下降能够导致NF-κB p65蛋白表达增加，进而促进外周血单个核细胞释放IL-1β、IL-6、IFN-γ和TNF-α等炎症因子，加重炎症反应程度，SHP-1是深静脉血栓治疗的潜在靶点。
SHP-1 regulates NF-κB to affect the levels of inflammatory factors in deep vein thrombosis
[Objective]To investigate the effect of SHP-1 and NF-κB on serum inflammatory factors in patients with deep vein thrombosis.[Method]Immunohistochemistry and Western Blot were used to analyze the expression level of SHP-1 in the vascular tissue of lower extremity veins.Peripheral blood mononuclear cells from patients with DVT were divided into three groups:control group,pcDNA NC group and pcDNA SHP-1 group.The concentrations of IL-1 β,IL-6,IFN-γ and TNF-αsecreted by peripheral blood mononuclear cells were analyzed by enzyme-linked immunosorbent assay.The protein levels of SHP-1 and NF-κB p65 in peripheral blood mononuclear cells of different experimental groups were detected by Western Blot.[Result]The results of immunohistochemistry showed that the expression of SHP-1 was decreased(P＜0.05),and the expression of NF-κB p65 was increased(0.21±0.01 vs 0.82±0.05;P＜0.05)in the vascular tissues of patients with deep vein thrombosis.The results of ELISA showed that the release levels of inflammatory factors IL-1β,IL-6,IFN-γ and TNF-α in the pcDNA SHP-1 group were decreased(P＜0.05).The results of Western Blot showed that the expression level of SHP-1 protein in the pcDNA SHP-1 group was increased(0.19±0.02 vs 0.22±0.03 vs 0.82±0.06;P＜0.05),while the expression level of NF-κB p65 protein was decreased(0.71±0.03 vs 0.73±0.05 vs 0.25±0.02;P＜0.05).[Conclu-sion]In patients with DVT,the decreased expression of SHP-1 can lead to the increased expression of NF-κB p65 protein,and then promote the release of inflammatory factors such as IL-1 β,IL-6,IFN-γ and TNF-α from peripheral blood mono-nuclear cells,and aggravate the degree of inflammatory response.SHP-1 was a potential target for the treatment of DVT.
SHP-1NF-κBdeep vein thrombosisinflammationserumimmunohistochemistryperipheral bloodfrac-ture of bone
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