Effects of rhizobium HH103 regulatory factor NolR on soybean nodule fixation and transcriptome difference analysis
[Objective]To investigate the effect of NolR in Sinorhizobium fredii HH103 on nodule fixation in Heilongjiang soy-bean and its symbiotic mechanism.[Method]Rhizobium HH103 and its knockout mutant △nolR were inoculated on 9 main soy-beans planted in Heilongjiang Province and Willams82 for pot experiment.The roots of soybean Heike 60 inoculated with HH103 and the mutant △nolR were selected for transcriptome sequencing.[Result]Compared with CK without bacteria,HH103 and △nolR can promote nodulation to some extent,but the nodulation effect of different varieties is different.Compared with inoculation with HH103,△nolR significantly reduced the nodule number and nitrogenase activity of Heike 60 by 46.47％and 15.76％,respectively.Transcriptome sequencing of the roots of Heike 60 showed that there were 661 differentially ex-pressed genes between the roots inoculated with HH103 and those inoculated with △nolR,including 538 up-regulated genes and 123 down-regulated genes.Gene nolR affects the expression of functional genes in the metabolic process,cellular anatomi-cal entity,catalytic activity and other processes in Heike 60.In addition,the absence of nolR gene also led to the enrichment of differentially expressed genes in phenylpropanoid biosynthesis,isoflavone biosynthesis.[Conclusion]The absence of nolR can inhibit the nodulation of Heike 60,which may affect the nitrogen-fixing nodulation of Heike 60 by regulating the signal synthe-sis pathway related to nodulation factors in plants.This study provides a theoretical basis for further investigation of the influ-ence of nolR gene on the mechanism of rhizobia-soybean symbiosis.
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