根瘤菌HH103调节因子NolR对大豆结瘤固氮影响及转录组差异分析
Effects of rhizobium HH103 regulatory factor NolR on soybean nodule fixation and transcriptome difference analysis
毕宇 1朱加楠 1刘春燕 2张悦 1田爽 1孙颖琦 1甄涛 1于德水3
作者信息
- 1. 黑龙江省科学院微生物研究所,黑龙江哈尔滨 150010
- 2. 黑龙江省科学院,黑龙江哈尔滨 150001
- 3. 黑龙江省科学院微生物研究所,黑龙江哈尔滨 150010;黑龙江省科学院,黑龙江哈尔滨 150001
- 折叠
摘要
[目的]探究费氏中华根瘤菌HH103中调节因子NolR对黑龙江大豆结瘤固氮的影响与共生机制.[方法]将根瘤菌HH103及其敲除突变体△nolR接种于9种黑龙江省主栽大豆和Willams82进行盆栽实验,并选取了接种HH103及突变体△nolR的黑科60大豆根部进行转录组测序.[结果]与不接菌的CK相比,接种HH103和△nolR在一定程度上都能促进结瘤,但是不同品种的结瘤效果不同.与接种HH103相比,△nolR使黑科60的根瘤数与固氮酶活性分别显著降低了 46.47％、15.76％.通过对黑科60根系进行转录组测序发现,接种HH103与接种△nolR的根系存在661个差异基因,其中上调表达基因538个,下调表达基因123个.nolR基因影响了黑科60中代谢过程、细胞解剖实体、催化活性等过程的功能基因表达,除此之外,nolR基因的缺失也使差异表达基因富集于苯丙素生物合成、异黄酮生物合成等途径.[结论]nolR的缺失会抑制黑科60的结瘤,这可能是通过调控植物与结瘤因子相关信号合成途径来影响黑科60的固氮结瘤,该研究为后续深入探究nolR基因对根瘤菌-大豆共生机制的影响提供了理论依据.
Abstract
[Objective]To investigate the effect of NolR in Sinorhizobium fredii HH103 on nodule fixation in Heilongjiang soy-bean and its symbiotic mechanism.[Method]Rhizobium HH103 and its knockout mutant △nolR were inoculated on 9 main soy-beans planted in Heilongjiang Province and Willams82 for pot experiment.The roots of soybean Heike 60 inoculated with HH103 and the mutant △nolR were selected for transcriptome sequencing.[Result]Compared with CK without bacteria,HH103 and △nolR can promote nodulation to some extent,but the nodulation effect of different varieties is different.Compared with inoculation with HH103,△nolR significantly reduced the nodule number and nitrogenase activity of Heike 60 by 46.47％and 15.76％,respectively.Transcriptome sequencing of the roots of Heike 60 showed that there were 661 differentially ex-pressed genes between the roots inoculated with HH103 and those inoculated with △nolR,including 538 up-regulated genes and 123 down-regulated genes.Gene nolR affects the expression of functional genes in the metabolic process,cellular anatomi-cal entity,catalytic activity and other processes in Heike 60.In addition,the absence of nolR gene also led to the enrichment of differentially expressed genes in phenylpropanoid biosynthesis,isoflavone biosynthesis.[Conclusion]The absence of nolR can inhibit the nodulation of Heike 60,which may affect the nitrogen-fixing nodulation of Heike 60 by regulating the signal synthe-sis pathway related to nodulation factors in plants.This study provides a theoretical basis for further investigation of the influ-ence of nolR gene on the mechanism of rhizobia-soybean symbiosis.
关键词
大豆/根瘤菌/结瘤固氮/调节因子NolR/转录组/差异基因表达/共生关系/农艺性状Key words
soybean/rhizobium/nodule fixation/regulatory factor NolR/transcriptome/differentially expressed gene/symbi-otic relationship/agronomic trait引用本文复制引用
出版年
2024
NETL
NSTL
万方数据