艾塞那肽改善糖尿病小鼠症状的核心基因探究

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中文摘要：[目的]探究艾塞那肽治疗糖尿病的机制和靶基因。[方法]下载数据集筛选差异表达基因，构建疗效相关的共表达网络筛选加权基因，选取两组数据交集基因并使用蛋白互作网络确立核心基因，使用糖尿病模型小鼠验证核心基因。[结果]共获取15个交集基因，综合GO/KEGG功能分析以及cytoHubba评分，选择血清和糖皮质激素诱导的激酶-1(serum and glucocorticoid regulated protein kinase-1，SGK1)作为核心基因。治疗组小鼠 7 w 空腹血糖和体重[(19。03±3。20)mmol/L;(48。23±1。97)g]较安慰剂组[(25。62±2。01)mmol/L;(53。23±1。79)g]均降低(P＜0。05);治疗组小鼠胰腺SGK1 mRNA和蛋白表达较安慰剂组降低(P＜0。05)，免疫组织化学染色显示治疗组小鼠胰腺组织结构相对完整，胰岛形态基本规则，较安慰剂组有明显改善，与生物信息学分析一致。[结论]艾塞那肽可通过抑制核心基因SGK1基因表达改善糖尿病小鼠血糖水平，保护胰腺组织。

外文标题：Exploring the core hub gene targeted by exenatide in improving symptoms of diabetes mellitus mice

外文摘要：[Objective]To explore the potential molecular mechanisms and hub gene of exenatide in diabetes mellitus(DM).[Method]The study harnessed datasets and selected differentially expressed genes(DEGs),then calculated the weighted genes by construction of a co-expression network correlating to drug intervention,and screened the intersection genes in both groups.The core hub gene was identified via protein-protein interaction(PPI)network and this assumption was validated in DM mice.[Result]Bioinformatics analyses yielded 15 intersecting genes,through integrated GO/KEGG functional analysis and cytoHubba scoring,serum and glucocorticoid regulated protein kinase(SGK1)was selected as the core gene.Exenatide-trea-ted DM mice exhibited significant reductions in blood glucose and body weight[(19.03±3.20)mmol/L and(48.23±1.97)g,respectively]compared with those of the placebo group[(25.62±2.01)mmol/L and(53.23±1.79)g,respectively]at the 7th week,and the difference was statistically significant(P＜0.05).The experimental group also demonstrated notable downregulation in both mRNA and protein expression of SGK1(P＜0.05).Immunohistochemistry staining exhibited pancreatic tissue structure was relatively intact and the morphology of islets was basically normal in treatment group compared to the place-bo group.The animal experiment results were consistent with bioinformatic predictions.[Conclusion]Exenatide can improve blood glucose level and protect pancreatic tissue in DM mice by inhibiting the expression of core hub gene SGK1.

外文关键词：

exenatideType 2 diabetes mellitusbioinformaticshub geneweighted gene co-expression network analysisser-um and glucocorticoid regulated protein kinase-1gene ontologydifferentially expressed genes

作者：

程优、刘影、房敬尧、甄若楠、朱黎娜、牛文彦

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作者单位：

天津医科大学朱宪彝纪念医院检验科/天津市内分泌研究所/国家卫健委激素与发育重点实验室/天津市代谢性疾病重点实验室,天津 300134

河北国际旅行卫生保健中心/石家庄海关口岸门诊部,河北石家庄 050091

天津中医药大学第二附属医院检验科,天津 300250

关键词：

艾塞那肽 2型糖尿病生物信息学枢纽基因血清和糖皮质激素诱导的激酶-1 加权基因共表达网络分析基因本体论差异表达基因

出版年：

2024

DOI：

10.16519/j.cnki.1004-311x.2024.06.0104

生物技术

黑龙江省微生物学会黑龙江省生物工程学会黑龙江省科学院微生物研究所

生物技术

CSTPCD

影响因子：0.611

ISSN：1004-311X

年,卷(期)：2024.34(6)