芬荠素诱发小鼠体内代谢的紊乱
Fengycin induces metabolic disorders in mice
徐海峰 1刘青 1张阳 2郑雨佳 2李宇健 1贺佳诺 1王一凡 1米婧璇 1赵雯雅 2杨雪 2赵山山 3郝光飞 3刘洪伟2
作者信息
- 1. 河北省科学院生物研究所,河北石家庄 050081;河北工程大学生命科学与食品工程学院,河北邯郸 056038
- 2. 河北省科学院生物研究所,河北石家庄 050081
- 3. 河北工程大学生命科学与食品工程学院,河北邯郸 056038
- 折叠
摘要
[目的]通过对喂养芬荠素小鼠脾的代谢组学分析,探讨芬荠素对小鼠脾组织代谢的影响.[方法]健康雄性SPF级C57小鼠16只,分为芬荠素处理组、对照组,经过8 w自由进食喂养后,取小鼠脾脏进行处理检测,运用高效液相色谱质谱联用仪检测,找出具有差异的特征代谢物及差异代谢通路.[结果]经代谢组学分析,与对照组相比,处理组共筛选出15种差异代谢物,显著影响了 6条代谢通路,包括组氨酸代谢、鞘脂代谢、甘油磷脂代谢、D-谷氨酰胺和D-谷氨酸代谢、精氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢等代谢通路.[结论]芬荠素干扰了小鼠体内多条代谢通路的平衡,降低了小鼠的抗炎症和抗氧化能力,对小鼠的机体健康造成了损伤.
Abstract
[Objective]The effects of fengycin on the metabolism of mouse spleen tissues were investigated by metabolomic a-nalysis of the spleens of fengycin-fed mice.[Method]Sixteen healthy male SPF-grade C57 mice were divided into the fengy-cin-treated group and the control group.After eight weeks of ad libitum feeding,the spleens of the mice were processed and detected by high performance liquid chromatography-mass spectrometry to identify the characteristic metabolites and metabolic pathways with differences.[Result]After metabolomics analysis,15 different metabolites were identified in the treated group compared with the control group,which significantly affected six metabolic pathways,including histidine metabolism,sphingolip-id metabolism,glycerophospholipid metabolism,D-glutamine and D-glutamate metabolism,arginine metabolism,and alanine,aspartic acid and glutamate metabolism.[Conclusion]Fengycin interfered with the homeostasis of multiple metabolic pathways in mice and reduced their anti-inflammatory and antioxidant capacity,causing damage to their organic health.
关键词
抗菌肽/芬荠素/高效液相色谱质谱联用技术/代谢组学/差异代谢物/差异代谢通路/机体免疫/能量代谢Key words
antimicrobial peptides/fengycin/HPLC-MS/metabolomics/differential metabolites/differential metabolic path-ways/organismal immunity/energy metabolism引用本文复制引用
出版年
2024
NETL
NSTL
万方数据