miR-612 regulates the metastatic activity of leukemia HL-60 cells by regulating FOXP1
[Objective]To investigate the effect of miR-612 and FOXP1 on the activity of acute myeloid leukemia cells.[Method]The expression levels of FOXP1 in normal and AML bone marrow samples were analyzed by immunohistochemistry.Human acute myeloid leukemia HL-60 cells were divided into four groups:miR NC group,miR-612 mimic group,si NC group and si FOXP1 group.Cell growth was measured by MTT assay.The expression levels of miR-612 and FOXP1 were de-tected by real-time fluorescence quantitative PCR.Cell migration ability was analyzed by Transwell assay.The expression level of FOXP1 was analyzed by Western Blot.The apoptosis rate was analyzed by flow cytometry.[Result]Compared with normal bone marrow samples,the expression level of miR-612 in bone marrow samples of AML patients decreased(P＜0.05),while the expression level of FOXP1 increased(P＜0.05).Compared with the miR NC group,the proliferation and metastasis ability of HL-60 cells in the miR-612 mimic group were significantly decreased,and the apoptosis rate was increased(P＜0.05).Compared with the si NC group,the proliferation and metastasis ability of HL-60 cells in the si FOXP1 group were significant-ly decreased,and the apoptosis rate was increased(P＜0.05).Luciferase reporter gene results showed that miR-612 could target and regulate the expression of FOXP1(P＜0.05).[Conclusion]Up-regulation of miR-612 expression can inhibit the proliferation and metastasis of HL-60 cells,and promote the apoptosis of HL-60 cells.In addition,the effect of miR-612 on HL-60 cells was related to the inhibition of FOXP1 expression.
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