Therapeutic Effect of Recombinant Collagen Gynecological Gel on Cervicitis in Rats
Abstract:Cervicitis is a kind of frequent gynecological disease,which brings trouble to more and more women.Antibiotics are of-ten used in clinical treatment of cervicitis,which has great side effects.In this study,recombinant collagen was developed into a topical gynecological gel and applied to the treatment of cervicitis in rats.25%phenol glue was used to induce cervicitis animal model in SD rats,and then recombinant collagen gynecological gel was injected into vagina by local administration for treatment.The changes of vulva swelling and purulent secretion in cervicitis model rats were observed.Hematoxylin-eosin(HE)and masson staining were used to examine the histopathologic changes and collagen deposition in rats.The expression levels of IL-6 protein and Ki-67 protein in the tissues were detected by immunohistochemical experiments.The regulation of inflammatory response and promoting cell proliferation were evaluated in the vaginal and cervical tissues of each group.Results showed that,compared with nor-mal group,the body weight of rats in model group was significantly reduced(P<0.05).Vaginal opening was red,swollen,and pu-rulent secretions flowed out(P<0.05).Cervical tissues showed cell degeneration,necrosis and exfoliation,and a large number of inflammatory cells infiltrated.The expression of IL-6 protein was significantly up-regulated(P<0.05),and the expression of Ki-67 was decreased(P<0.05).Compared with model group,the body weight of rats in positive control group,GG01 group and GG02 group was significantly increased after treatment(P<0.05).The swelling and secretions of the vaginal opening were significantly re-duced(P<0.05),the inflammatory cell infiltration was significantly reduced,there was no obvious edema and congestion,collagen deposition and orderly arrangement were shown in the tissues,and the treatment effect of GG02 group was more significant.The expression levels of IL-6 and Ki-67 in GG02 group and positive control group were close to those in normal group,the inflamma-tory response was reduced,and the proliferation level of damaged tissue cells was restored.The recombinant collagen gynecologi-cal gel has a good therapeutic effect on acute cervicitis in rats and provides data support for its clinical application.
recombinant collagengynecological gelcervicitisanimal model
万方数据
维普
