Preservation Effect of Tissue Protectant on Viral Nucleic Acid and RNA of Guinea Pig Skin Tissue Samples
Viral nucleic acid and RNA of biological tissue samples are easy to be affected by external environment and easily degrade,resulting in serious deviations in experimental results.Taking viral nucleic acid and guinea pig skin tissue sample RNA as research objects,the protective effect of tissue protectant on viral load and RNA in guinea pig skin tissue sample after preservation of them at different temperatures and different times was explored.The results showed that after storage at 4℃for 30 days(d),25℃for 7 d or 37℃for 24 hours(h),the viral load stored in tissue protectants did not change significantly compared with the initial viral nucleic acid dose(P>0.05).After storage at 4℃for 30 d,25℃for 7 d or 37℃for 24 h,compared with liquid nitrogen storage group,both tissue protectant group and commercially available RNA later group could maintain the extraction amount and purity of sample RNA in guinea pig skin tissue,with no statistical significance(P>0.05).The results showed that the tissue protectant could be used as a storage solution for scientific research or clinical tissue samples.It was placed at 4℃,25℃and 37℃for a certain time,without affecting the viral load of viral nucleic acid and the amount and purity of RNA extraction from guinea pig skin tis-sue samples,which made the sample preservation more convenient and efficient.
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