首页|生物检测试剂保藏技术研究进展

生物检测试剂保藏技术研究进展

扫码查看
原文链接
  • 万方数据
  • 维普
生物检测试剂的扩增性能,尤其是酶试剂的生物活性对荧光定量PCR等的检测结果起决定性作用.采用不同的保藏技术对维持检测试剂的生物活性,实现生物检测试剂室温条件下长时间稳定保藏与非冷链运输,保证检测结果的稳定性、准确性、特异性和灵敏度等至关重要.针对低温保藏技术、真空冷冻干燥技术、喷雾干燥技术以及玻璃化冷冻技术4种不同保藏技术的研究进展进行了综述,在应用中总结优缺点,以期对后续相关工作的开展提供一定的科学依据.
Research Progress of Biological Detection Reagent Preservation Technology
The amplification performance of biological detection reagents,especially the biological activity of enzyme reagents,plays a decisive role in the detection results of real-time quantitative PCR.It is crucial to adopt different preservation technolo-gies to maintain the biological activity of detection reagents,achieve long-term stable preservation and non-cold chain transporta-tion of biological detection reagents at room temperature,and ensure the stability,accuracy,specificity and sensitivity of detec-tion results.This paper reviewed the research progress of four different preservation technologies,including vacuum freeze-drying technology,spray drying technology,vitrification freezing technology,and summarized the advantages and disadvantages in ap-plication,in order to provide a certain scientific basis for the follow-up related work.

biological detection reagentscryogenic preservation technologyvacuum freeze-drying technologyspray drying technologyvitrification freezing technology

刘莹莹、赵可心、王艺霏、路凯旋、胡孔新

展开 >

中国检验检疫科学研究院,北京 100176

生物检测试剂 低温保藏技术 真空冷冻干燥技术 喷雾干燥技术 玻璃化冷冻技术

国家重点研发计划项目北京市科技计划项目

2021YFC2600503Z221100005222007

2024

10.19586/j.2095-2341.2023.0110

生物技术进展
中国农业科学院茶叶研究所 中国农业科学院生物科技研究所

生物技术进展

CSTPCD
影响因子：0.554
ISSN：2095-2341
年,卷(期)：2024.14(1)
  • 9