生物技术进展2024,Vol.14Issue(1) :94-101.DOI:10.19586/j.2095-2341.2023.0110

生物检测试剂保藏技术研究进展

Research Progress of Biological Detection Reagent Preservation Technology

刘莹莹 赵可心 王艺霏 路凯旋 胡孔新
生物技术进展2024,Vol.14Issue(1) :94-101.DOI:10.19586/j.2095-2341.2023.0110

生物检测试剂保藏技术研究进展

Research Progress of Biological Detection Reagent Preservation Technology

刘莹莹 1赵可心 1王艺霏 1路凯旋 1胡孔新1
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作者信息

  • 1. 中国检验检疫科学研究院,北京 100176
  • 折叠

摘要

生物检测试剂的扩增性能,尤其是酶试剂的生物活性对荧光定量PCR等的检测结果起决定性作用.采用不同的保藏技术对维持检测试剂的生物活性,实现生物检测试剂室温条件下长时间稳定保藏与非冷链运输,保证检测结果的稳定性、准确性、特异性和灵敏度等至关重要.针对低温保藏技术、真空冷冻干燥技术、喷雾干燥技术以及玻璃化冷冻技术4种不同保藏技术的研究进展进行了综述,在应用中总结优缺点,以期对后续相关工作的开展提供一定的科学依据.

Abstract

The amplification performance of biological detection reagents,especially the biological activity of enzyme reagents,plays a decisive role in the detection results of real-time quantitative PCR.It is crucial to adopt different preservation technolo-gies to maintain the biological activity of detection reagents,achieve long-term stable preservation and non-cold chain transporta-tion of biological detection reagents at room temperature,and ensure the stability,accuracy,specificity and sensitivity of detec-tion results.This paper reviewed the research progress of four different preservation technologies,including vacuum freeze-drying technology,spray drying technology,vitrification freezing technology,and summarized the advantages and disadvantages in ap-plication,in order to provide a certain scientific basis for the follow-up related work.

关键词

生物检测试剂/低温保藏技术/真空冷冻干燥技术/喷雾干燥技术/玻璃化冷冻技术

Key words

biological detection reagents/cryogenic preservation technology/vacuum freeze-drying technology/spray drying technology/vitrification freezing technology

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基金项目

国家重点研发计划项目(2021YFC2600503)

北京市科技计划项目(Z221100005222007)

出版年

2024
生物技术进展
中国农业科学院茶叶研究所 中国农业科学院生物科技研究所

生物技术进展

CSTPCD
影响因子:0.554
ISSN:2095-2341
参考文献量9
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