Development of Sheep-derived Genomic DNA Reference Material
Abstract:In recent years,food safety incidents such as adulteration of mutton and its meat products have been occurring,and in order to improve the legal basis for market enforcement and inspection,a standard substance for sheep genomic DNA has been developed by using digital PCR to quantify the sheep HELZ gene.Since the standard substance can be used to measure the accu-racy of detection methods,it can determine the adulteration of mutton in food and related products.In this research,the standard material of sheep genome DNA was developed as the positive control for the detection of mutton in food and related products.The uniformity and stability of the reference materials were evaluated by digital PCR.The results showed that the reference materials had good uniformity and could be stably stored at 4℃and 25℃for 14 days,and at-20℃for 6 months.The standard values of sheep-derived genomic DNA reference material(high concentration)and sheep-derived DNA reference material(low concentra-tion)and their extended uncertainties were(5.44±0.45)×103 copies·μL-1 and(5.68±0.54)×102 copies·μL-1,respectively,which were jointly determined by nine different laboratories.The development of sheep-derived genomic DNA reference materials pro-vides a technical basis for the development of animal-derived reference materials,and will establish a technical platform for the prepa-ration,quantitative detection,quality control and quantitative value traceability of sheep-derived genomic DNA reference materials.
reference materialHELZ gene of sheep-deriveddigital PCR
万方数据
维普
