Study on Optimization of Culture Method and Improvement of Sleep Function of Limosilactobacillus reuteri HCS02-001
In order to optimize the cultivation method of Limosilactobacillus reuteri HCS02001 and explore its sleep promoting func-tion,the optimal cultivation was screened through single factor experiment and response surface experiment based on the γ-aminobu-tyric acid(GABA)yield,and its sleep promoting function was evaluated through mouse experiment.Under the condition of that culture temperature was 37℃,the culture time was 24 h and the initial pH was 6.5,the GABA yield of L.reuteri HCS02-001 was 5.58 g·L-1.No direct sleep phenomenon occurred in mice in the direct sleep experiment.The experimental results of prolonging the sleep time of mice hypnotized by pentobarbital sodium showed that the mice in the high-dose group effectively prolonged the sleep time,and the re-sults of pentobarbital sodium subthreshold dose hypnosis experiment showed that the sleep rate of mice in the high-dose group was as high as 40%.Barbiturate sleep latency experiments showed that mice in the high-dose group had reduced sleep latency and could act synergistically with barbiturate sodium.The results showed that L.reuteri HCS02-001 had the function of sleep aid.
Limosilactobacillus reuterisingle factorresponse surfacesleep aid