Bioinformatics Analysis on the Regulation Mechanism of NHP2 on Hepatocel-lular Carcinoma Senescence
To investigate the expression of ribonucleo protein NHP2 in hepatocellular carcinoma(HCC)and to explore the relationship with disease progression and prognosis.Differentially expressed genes of HCC were screened by Quick Go and GEPIIA 2 online databases,and NHP2 was identified as object of study.Furthermore,bioinformatics methods such as STRING,TIMER 2.0,UALCAN database were used to analyze the differential expression of NHP2 in pancreatic cancer and its correlation with pathological progress in HCC and other pancreatic cancers,and to predict the relationship between prognosis and survival.The target miRNAs and lncRNAs were analysed with the miRNet database,the possible regulatory network of CeRNAs was mapped by Cytescape v3.8.0.Results showed that,113 genes related to cell aging and 2 206 differ-entially expressed genes(DEGs)in HCC were found in the online database.19 DEGs were involved in the biological process of cell aging in HCC.Among them,NHP2 is significantly highly expressed in a variety of cancers including HCC,and the prognosis of HCC with high NHP2 expression is poor in statistical difference.There are 10 interacting proteins encoded by the NHP2 gene,it is mainly involved in 1 signalling pathway(KEGG),6 molecular functions(MF),11 cellular components(CC)and 7 biological processes(BP).Two NHP2-targeting miRNAs and 57 NHP2-targeting lncRNAs had been predicted.The results indi-cated that,NHP2 is highly expressed in a variety of tumors,including HCC,and is significantly different in age and stage of patients with a variety of tumors.During the proliferation and senescence of HCC,it may be regulated by the regulatory axis of lncRNAs/miR-1-3p/NHP2 or lncRNAs/miR-124-3p/NHP2 and be a prognostic predictor and a potential therapeutic target of HCC.
万方数据
维普
