Comparative Analysis of Chloroplast Genomes from Rubus poliophyllus and Rubus yunanicus
Rubus poliophyllus and R.yunanicus belong to the Rubus subsect.Acuminati(Focke)T.T.Yu&L.T.Lu of Rosaceae.They have similar morphological characteristics and geographical distribution.So it is difficult to distinguish them only by mor-phological characteristics.In this study,their chloroplast genome sequence were obtained by high-throughput sequencing tech-nology of Illumina,and the sequences were filtered,assembled,annotated and analyzed.Then the phylogenetic tree based on chloroplast genome sequence was constructed.The results showed that the complete chloroplast genomes size of R.poliophyllus and R.yunanicus were 156 298 bp and 156 299 bp,and the GC content of the two species was 37.2%.One hundred and fifty-nine chloroplast genes were detected,including 104 protein-coding genes,45 tRNA genes and 10 rRNA genes respectively.The number of palindromic repeats,direct repeats,reverse repeats between R.poliophyllus and R.yunanicus were 17,17;19,17;4,3,respectively.There were 32 codons with relative synonymous codon usage(RSCU)>1.00 in the these two species.The co-don preference ended with A/U,and the effective number of codons were greater than 45.Phylogenetic analysis based on the chloroplast geneme and the rpoC1-rpoB gene sequence showed that R.poliophyllus and R.yunanicus were clustered into the same clade,which indicated that they had a closely genetic relationships.This study will provide a theoretical basis for the molecular identification,genetic diversity and phylogenetic relationships of R.poliophyllus and R.yunanicus.
万方数据
维普
