Optimization of Preparation Technology of Lactiplantibacillus plantarum HCS03-001 Freeze-dried Powder and its Anti-Helicobacter pylori Function
The aim of the study is to optimize the preparation technology of freeze-dried powder of Lactiplantibacillus plantarum HCS03-001 and explore its functional effect of anti-Helicobacter pylori.In this study,the preparation technology of freeze-dried powder of Lactiplantibacillus plantarum HCS03-001 was optimized by single factor test,principal factor analysis and response surface test,and then the anti-Helicobacter pylori effect of the probiotics was verified by bacteriostatic test in vitro.The results showed that the optimum conditions for preparing freeze-dried powder were fermentation temperature 37℃,fermentation time 16 h,inoculum 5.2%and freeze-drying time 44 h.Under these conditions,the number of live bacteria was 5.85×1011 CFU·g-1.These results of bacteriostatic test in vitro showed that the fermentation supernatant and bacterial suspension of Lactiplantibacil-lus plantarum HCS03-001 could effectively inhibit the growth of Helicobacter pylori,and the inhibition rate was 69.55%～78.78%,which showed that Lactiplantibacillus plantarum HCS03-001 have the function of anti-Helicobacter pylori.
万方数据
维普
