The Mechanisms of IL-17 Inhibiting Pseudomonas aeruginosa Infection on Lung Epithelial Cells
Pseudomonas aeruginosa is a common pathogen.Interleukin-17(IL-17)family cytokines are directly involved in the host immune response,which are key mediators of the defense against infection or inflammation in host.Using recombinant hu-man IL-17 protein to treat Pseudomonas aeruginosa PAO1 and explore its invasive effect on lung epithelial cell A549,it was found that IL-17 can inhibit the invasion of PAO1 to A549 cells without affecting bacterial growth and cell proliferation.To ex-plore the mechanism of IL-17 influencing PAO1 infection on host cells,we utilized transcriptome sequencing to analyze the changes of IL-17 on the PAO1 transcriptome.The results showed that there are 210 upregulated genes and 123 downregulated.GO functional analysis showed that the differential genes were mainly enriched in peptidase activity,protein binding and proteol-ysis.KEGG enrichment analysis showed that the differential genes were involved in RNA degradation,biofilm formation,micro-bial metabolism,ABC transport and carbon metabolism.In addition,the biofilm formation experiments further confirmed that IL-17 could reduce PAO1 biofilm formation.These findings provided a new idea and theoretical basis for the treatment of drug-resis-tant Pseudomonas aeruginosa in the treatment of related diseases caused by clinical infection.
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