Construction of Prognostic Prediction Model of Breast Cancer Based on Tumor-associated Fibroblast Genes and Analysis of Immune Infiltration
Metastasis and malignant progression of breast cancer are deeply related to the tumor microenvironment.Tumor-associ-ated fibroblasts(CAFs)are comparatively important cells in the tumor microenvironment which have implications on tumor pro-gression and treatment.We obtained single-cell sequencing data of breast cancer downloaded from gene expression omnibus data-base,clustered the cells of tumor microenvironment,and then used WGCNA to identify the key genes related to CAF,and con-structed a risk score model with the genes in TCGA-BRCA database,and performed survival analysis,Cox regression analysis,ROC curves,and constructed a column line graph to predict the performance of the model.Model-related pathways were analyzed by GO and KEGG.The relationship between risk score and clinical features and tumor microenvironment was explored by somatic mutation,immune infiltration analysis,stemness index analysis,and drug sensitivity analysis.A prognostic prediction model based on 10 CAF genes was constructed and validated in accordance with the risk scores.Patients were classified into high-and low-risk groups according to the risk scores,and the prognosis of patients in the high-risk group was worse,and the column plot and ROC curve also showed that the model had a good predictive efficiency,and the immune infiltration level of patients with breast cancer in the high-risk group was lower and the stemness index was higher,and the patients in the high-risk group were more sensitive to 2 drugs,namely,paclitaxel and lapatinib.Patients in the high-risk group had a lower level of immune infiltra-tion and a higher dryness index,and patients in the high-risk group had a higher sensitivity to paclitaxel and lapatinib.The risk scores of the 10 CAF-related genes can individually predict the progress and therapeutic effects of breast cancer,which provides ideas for clarifying the mechanism of the role of CAF-related genes in breast cancer and provides a theoretical basis for the clini-cal individualization of treatment for patients with breast cancer susceptibility gene.
breast cancercancer associated fibroblaststumor mutcotion bursenprognostic modelsimmune infiltration
万方数据
维普
