Advances in Emerging and Improved Methods for Detecting Viral Titers
Virus titration is an important analytical method in the biopharmaceutical industry,which is widely used in the devel-opment and production of viral biological products,the validation of virus clearance and inactivation processes,and the detec-tion of exogenous viruses to ensure the activity and effectiveness of viral biologics,as well as the viral safety of biological prod-ucts.It is important to establish a fast,simple,and accurate method for the detection of virus titration.This article summarized the characteristics,principles and specific applications of the traditional,emerging and improved methods for detecting viral ti-ter and compared their advantages and disadvantages.Some emerging methods,such as droplet digital PCR,viral quantitative capillary electrophoresis,in situ hybridization(ISH)assay with chemiluminescent detection,laser force cytology,etc.,have im-proved the shortcomings of traditional methods such as time-consuming and labor-intensive,poor repeatability,low accuracy,and large subjectivity of results,and have achieved the advantages of rapidity,sensitivity,high degree of automation,high preci-sion,and more robust and objective results.However,some new methods are expensive or not widely used,and it is necessary to select the appropriate virus titration method according to the experimental purpose.
万方数据
维普
