The single specificity of ordinary PCR primers for amplifying target genes limits the universality in clinical disease de-tection,while multiplex PCR needs to avoid interference of secondary structures between several primer pairs,which also limits its application.The degenerate primers based on the conserved region design of genes can detect highly similar species,and de-generate PCR shows good efficiency,sensitivity,specificity and low cost in the application of correlation detection.Currently,degenerate PCR technology has been widely used in mutiple fields.This article reviewed the application of degenerate PCR tech-nology in clinical,food safety,animal and plant diseases,parasites,aquaculture and other fields,and discussed its advantages and disadvantages,in order to provide reference and guidance for the further development of degenerate PCR and its application in other fields.
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