The Effect of Chemical Reagents on the Efficiency of Gene Editing in Aspergillus tubingensis
Some chemical reagents can improve gene editing efficiency by regulating cell cycle or intracellular DNA repair pathways.Using Aspergillus tubingensis as the research material,the gene fwn related to spore pigment synthesis was edited by CRISPR/Cas9 tool.Eight chemical reagents were added during the transformation process.Finally,the effects of eight chemical reagents on the gene editing efficiency of Aspergillus tubingensis were detected by spore color statistics and resistance gene PCR verification.The results showed that,compared with the control group,adding benomyl,RS-1,vanillin and chlorpromazine in the process of pro-toplast transformation,the homologous recombination efficiency reached 85.45%,60.90%,59.45%and 65.95%,respectively,which was 18.05%,9.35%,7.9%and 14.4%higher than that of the control group.When benomyl was used in combination with other reagents,the homologous recombination efficiency was further improved,up to 97.92%.Therefore,the application of chemical reagents in protoplast transformation provides a new idea for improving the gene editing efficiency of filamentous fungi.
chemical reagentshomologous recombination efficiencyAspergillus tubingensis
万方数据
维普
