Study on Plasma Metabolic Changes in Children with OSA Based on Gas Chromatography-mass Spectrometry Detection
Plasma metabolite changes in children with obstructive sleep apnea(OSA)were studied by gas chromatography-mass spectrometry(GC-MS)to find candidate metabolic markers and diagnostic methods for OSA in children.Five children diagnosed with OSA in our hospital and seven healthy children were selected.After polysomnography(PSG)examination,3 mL of venous blood was withdrawn for GC-MS metabolomics analysis in the early morning of the next day to identify and analyze the differential metabolites and enrich the corresponding metabolic pathways.The results revealed that a total of 105 potential differential metab-olites were screened in children with OSA compared with healthy controls,with 65 up-regulated and 40 down-regulated expres-sions.The differential metabolites were enriched in 29 metabolic signaling pathways,of which 12 were significant different,including alanine,aspartate and glutamate metabolism,citrate cycle,tryptophan metabolism,histidine metabolism,glycolate and dicarboxylic acid metabolism,and phenylalanine,tyrosine and tryptophan biosynthesis,etc.Plasma levels of amino acids,fatty acids,and carbohydrates were significantly altered in children with OSA,and changes in these metabolites may be a poten-tial mechanism for the pathogenesis of children with OSA.
万方数据
维普
