Effects of Hypoxic Exercise on the Expression of Serum Exosome Wnt3a in Obese Rats
Currently,obesity stands as a worldwide epidemic.Research has found that exercise training could effectively reduce body weight and enhances lipid metabolism in obese rats,with Wnt/β-catenin signaling pathway playing a pivotal role,especial-ly Wnt3a.However,the mechanism underlying of influence Wnt3a in adipose tissue remains unclear.This study aimed to delve into the expression alterations of serum exosome Wnt3a in nutritionally obese rats following exercise training under varying hypox-ic conditions,ultimately contributing to the theoretical framework of lipid metabolism modulated by hypoxic training.A total of forty obese rats were randomly assigned into four groups(n=10 each):normoxic exercise group(CE),normoxic quiet group(C),hypoxic exercise group(HE),and hypoxic quiet group(H).After a four-week hypoxic exercise intervention,the body weight,perirenal fat weight,and body length were recorded.The serum levels of low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),total cholesterol(TC),and triglyceride(TG)were determined using a lipid bio-chemical detection kit.Subsequently,serum exosomes were isolated and subjected to proteomic analysis.The findings revealed that hypoxic training significantly reduced body weight,fat-body ratio,Lee's index,TC,TG,and LDL-C levels(P<0.05),while increasing HDL-C(P<0.05)in obese rats.TEM electron microscopy distinctly depicted the extracellular vesicle-like struc-ture of exosomes in groups C,H,CE,and HE,and WB detection of exosomes confirmed the presence of CD9,CD63 and TSG101 bands.Notably,the WB analysis showed no significant difference in Wnt3a protein expression between simple hypoxia and exercise alone.However,hypoxia significantly elevated Wnt3a protein expression.All three intervention methods contribut-ed to improved blood lipid profiles in obese rats,with hypoxic training exhibiting a more profound effect.Consequently,hypoxic training increased the level of Wnt3a in serum exosomes.
hypoxia trainingWnt3asignaling pathwaylipid-lowering weight loss
万方数据
维普
