Control of Tobacco Bacterial Wilt by Bacillus velezensis Combined with Calcium-containing Soil Conditioner
Tobacco is one of the most important cash crops,and bacterial wilt of tobacco causes a great loss to tobacco produc-tion.Therefore,a safe and effective method is needed to prevent the occurrence of tobacco bacterial wilt.In the previous study,it found that the soluble calcium fertilizer could effectively control tobacco bacterial wilt.When tobacco was planted in the field,the best combination was selected by applying different soil conditioner containing calcium and biocontrol bacteria.The quantity of Ralstonia solanacearum was quantified through fluorescence quantitative PCR.The composition and structure of microbial com-munity in tobacco rhizosphere soil under different treatments were studied by high-throughput sequencing.Field experiments showed that the application of soil conditioner and biocontrol agent could effectively reduce the occurrence of tobacco bacterial wilt,but only ammonium calcium nitrate could compound with biocontrol bacteria GT11 better.The combination treatment de-creased the quantity of bacterial wilt in soil,improved the composition and structure of microbial community in tobacco rhizo-sphere soil,and significantly reduced the incidence and disease index of tobacco bacterial wilt.The control effect in the field was up to 60%.The results showed that the combination of calcium ammonium nitrate and Bacillus velezensis GT11 could effectively control tobacco bacterial wilt,which provided a new idea and theoretical basis for tobacco production.
calcium ammonium nitrateBacillus velezensistobacco bacterial wiltbiological control
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