In vivo Study of p,p'-DDT Bioconcentration Kinetics in Chinese Mitten Crab Eriocheir sinensis
To investigate the distribution characteristics and bioconcentration kinetics of p,p'-dichlorodiphenyltri-chloroethane(p,p'-DDT)in Chinese mitten crab Eriocheir sinensis,the two-compartment kinetic model was employed.Using the semi-static water exposure method,the study conducted dynamic monitoring of p,p'-DDT concentrations in the muscle,gonad,and hepatopancreas of E.sinensis during both bioconcentration and depuration phases.Non-linear fitting was applied to these monitoring results to obtain various bioconcentration kinetic parameters for each tissue and organ,including first order constant for uptake(k1),first order rate constant for depuration(k2),bioconcentration factor(BCF),concentration in the crab at steady-state(CA-SS)and biological half-life(t50).Results among various tissues,k1 was 0.021～16.039,k2 was 0.102～0.191,BCF was 0.146～83.974,CA-SS was 37.50～19 423.50 μg·kg-1,t50 was 3.63～6.80 d.Goodness-of-fit tests indicated that the bioconcentration and depuration processes of p,p'-DDT in muscle,gonad and hepatopancreas conformed to the two-compartment kinetic model while E.sinensis exposed to both 25 and 500 μg·L-1 p,p'-DDT.In details,k1 and BCF in muscle,gonad and hepatopancreas decreased along with the increase of p,p'-DDT concentration.In contrast,CA-SS increased as the concentration raised.Moreover,k2 and t50 displayed no significant correlation with the exposure concentration variation.As for the difference among tissues,the bioconcentration effect of p,p'-DDT in different tissues ranked as follows:hepatopancreas>gonad>muscle.
p,p'-DDTEriocheir sinensisbioconcentrationtwo-compartment kinetic model
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