Study on Isolation,Culture,Biological Characteristics and Applications of ADSCs
This study aims to perform primary isolation,culture,and identification of adipose-derived stem cells(ADSCs),with the objective of establishing a standardized method for the primary isolation,culture,and expansion of adipose stem cells for au-tologous transplantation therapy,providing a foundation for the transplantation treatment of rheumatoid arthritis.Three types of culture media,namely K-SFM,M-199,and DMEM-low glucose,were used to culture the isolated adipose stem cells,and com-parisons were made regarding cell morphology,passage,and recovery vitality.Flow cytometry was employed to detect cell sur-face factors including CD34,CD45,CD73,CD105,and HL-ABC,in order to determine the specificity and purity of the isolat-ed and cultured cells,as well as to compare whether there are differences in cell characteristics under different culture condi-tions.Chondrogenic and adipogenic induction experiments were conducted on cells under several culture conditions to assess and compare whether there are changes in stem cell characteristics between different treatments.The cells obtained through this meth-od,upon flow cytometry detection of cell surface factors,conformed to the surface antigen characteristics of adipose stem cells and exhibited a high degree of stem cell purity.Under different culture systems,the cells showed good adaptability,maintaining high cell vitality even after passage or cryopreservation and recovery.In the chondrogenic and adipogenic induction experiments,the cells demonstrated significant stem cell differentiation potential.According to this optimized method,the obtained stem cells,having passed quality inspection and confirmed to be free from foreign matter contamination,cross-contamination,and ge-netic variation risks,while maintaining high vitality and distinct stem cell characteristics,can be utilized for basic research on autologous stem cell transplantation for the treatment of rheumatoid arthritis.
NETL
NSTL
万方数据
