Effects of siRNA Targeting to Interfere with the Expression of TRAF6 on the Proliferation and Apoptosis of Lung Cancer Cells
The study aimed to investigate the effect of interfering tumor necrosis factor receptor-associated factor 6(TRAF6)ex-pression on proliferation and apoptosis of lung cancer A549 cells in vitro and its possible mechanism.A549 cells were transfected with siRNA,and the mRNA and protein expression levels of TRAF6 were detected by fluorescence quantitative PCR(qPCR)and Western blottingrespectivly.Proliferation was detected by CCK-8 assay,apoptosis rate was detected by Annexin V-FITC/PI double staining flow cytometry,and invasion ability of transfected cells was investigated by Transwell chamber.Western blotting was used to detect TRAF6/TAK1 pathway related proteins(TAK1,ub-TAK1,p-TAK1,Bax/Bcl-2,P-P65).After siRNA interference,the expression levels of TRAF6 mRNA and protein in A549 cells were significantly decreased compared with the control group,cell proliferation was reduced,apoptosis was increased,and invasion ability was decreased significantly.Meanwhile,interference with TRAF6 expression inhibited TAK1 activation,significantly induced Bax expression,and significantly inhibited Bcl-2 and P-P65 protein expression.Thus,down-regulation of TRAF6 expression can inhibit the proliferation and invasion of lung cancer cells and promote apoptosis,and its mechanism may be related to the inhibition of TRAF6/TAK1 signaling pathway activation.
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