Progress on Flammulina filiformis Transformation Mediated by Agrobacterium tumefaciens
Flammulina filiformis,a famous medicinal and edible mushroom fungus in china,has a good prospect for develop-ment and application.An efficient and stable genetic transformation system is a key technology for studying its gene function.In currently,Agrobacterium-mediated gene transferring is one of the major methods in genetic transformation of F.filiformis.In this paper,key factors involved in Agrobacterium-mediated genetic transformation of F.filiformis incluing recipient tissue,Agrobacte-rium strains,binary vectors,acestosyringone,and co-culture time were systematically reviewed.The progress of Agrobacteria-mediated transformation technology in the study of gene function of F.filiformis was summarized,and the existing issues and ap-plication prospects of this technology were briefly discussed.The aim of this study is to provide a reference for the establishment of an efficient Agrobacterium-mediated genetic transformation system of F.filiformis for the future.
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