Optimization of Liquid Fermentation Conditions for Mycelial Biomass and Exopolysaccharide Production of Simplicillium lanosoniveum
Simplicillium lanosoniveum has been reported to have a variety of biological effects,and has been traditionally used as a biological control agent.However,there were few reports on its liquid fermentation.In this study,a hyphomycetous fungus SL001 isolated from Stemonitis splendens was identified as S.lanosoniveum through morphology and phylogenetic analysis.Then,taking mycelial biomass and exopolysaccharides yield as indicators,the composition and proportion of fermentation medium were optimized through single-factor and orthogonal experiments to clarify the nutritional needs of liquid fermentation.The results showed that the highest mycelial biomass and exopolysaccharides yield were harvested under the nutritional conditions of 40 g·L-1 maltose,3 g·L-1 peptone and 1 g·L-1 KH2PO4,and the influence on mycelial biomass of S.lanosoniveum was maltose>peptone>KH2PO4.Finally,the culture conditions were further optimized based on nutritional needs.The results showed that the optimum liquid fermentation conditions were 28℃,pH 5.5,150 r·min-1,80 mL/250 mL and 6%inoculum size.The mycelial biomass and exopolysaccharides yield were as high as 1.69 g·100 mL-1 and 3.968 mg·mL-1 respectively,which were significantly differ-ent from the control(P<0.01).This work provides a theoretical basis for further research on antibacterial agent function and in-dustrial production of S.lanosoniveum.
S.lanosoniveumliquid fermentationnutritional factorsculture conditionsmycelial biomassexopolysaccharide production
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