Effect of Mineral Elements and Phytohormones on the Growth of Morchella exima
The effects of mineral elements and phytohormones on mycelial growth and extracellular polysaccharide production of Morchella were discussed.The Morchella spp.was used as the test material.The diameter of colony on the different media was used as the inspection index to study the effect of different concentrations of mineral elements and hormones on mycelial growth of Morchella mycelium.Then,from each substance,several preferred mass concentrations and configure the culture medium were selected according to these mass concentrations to culture Morchella esculenta,and measured the polysaccharide content was measured.Results showed that,CaSO4 in mineral elements played an important role in promoting the growth of Morchella es-culenta.The effect was most obvious when the mass concentration was 10 g·L-1.Low concentrations of MgSO4(<0.1 g·L-1)pro-moted the growth of Morchella.At the concentration of 0.01 g·L-1,the promotion effect was the strongest occured,and when the concentration was too high,the inhibition effect appeared and the inhibitory effect becomed more and more obvious with increas-ing concentration;salicylic acid had no obvious effect on the growth of Morchella.Methyl jasmonate at a suitable mass concentra-tion(0.005～0.050 mg·L-1)could promote the growth of Morchella mycelia.A certain concentration of CaSO4(about 10 g·L-1)could promote the production of extracellular polysaccharides from Morchella.The higher the mass concentration of MgSO4 was,the higher the total amount of extracellular polysaccharides of Morchella was.Appropriate concentration of CaSO4,MgSO4 and methyl jasmonate can promote the growth of Morchella mycelium,and salicylic acid had no effect on the growth of Morchella.CaSO4 has a dual effect on the production of extracellular polysaccharides from Morchella.it not only inhibits the production of ex-tracellular polysaccharides,but also promotes the production of extracellular polysaccharides.MgSO4 shows a promotion effect on the production of extracelluar poly saccharicles.
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