Isolation,Identification and Whole-genome Sequence Analysis of Phytophthora nicotianae Antagonistic Bacteria XC-29
Phytophthora nicotianae is a soil-borne oomycete that can cause black shank disease of tobacco,which is very harmful to tobacco production.A strain XC-29 with strong biocontrol function against P.nicotianae was screened,and its antagonistic me-tabolites and genes were mined by analyzing its genomic information.The antibacterial activity and control effect of biocontrol bacterium XC-29 were verified by the plate confrontation method and pot test.The strain was identified by morphological observa-tion and 16S rRNA amplicon sequencing.The antagonistic mechanism of strains was verified the disease prevention effect of the strain.The strains were identified by exploration by whole genome and transcriptomic sequencing.The results showed that the an-tagonistic strain XC-29 isolated from rhizosphere soil was identified as Bacillus safensis.Its whole genome sequencing predicted 136 carbohydrate-active enzymes and 11 gene clusters encoding the synthesis of secondary metabolites,of which six were identi-fied as the synthesis clusters of antimicrobial substances,encoding lichenysin,zwittermicin,schizokinen,fengycin,bacilysin and bacilibactin.The results of transcriptome sequencing further confirmed that the strain genes could encode corresponding anti-bacterial substances.In conclusion,Bacillus safensis XC-29 has a strong antagonistic effect on Phytophthora nicotianae.The whole genome and transcriptome analysis preliminarily revealed the disease inhibition mechanism of Bacillus safensis XC-29,which provides a theoretical basis for further exploration of the antibacterial mechanism and biological prevention and control of antagonistic bacteria.
tobacco black shankBacillus safensiswhole genome sequencingsecondary metabolites
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