一种低成本、高效、操作简便的转基因植株鉴定方法
A Low-cost,Efficient and Easy-to-operate Method for Transgenic Plant Identification
彭海霞 1汪世娟 2辛转霞 1梅莉 1马猛2
作者信息
- 1. 西北农林科技大学风景园林艺术学院,陕西 杨凌 712100
- 2. 西北农林科技大学生命科学学院,陕西 杨凌 712100
- 折叠
摘要
鉴于特异性PCR、试纸条等常用转基因植株检测方法存在费时费力且需要一定专业技术等局限性,研究希望探索一种低成本、高效、操作简便且适用于小麦全生育期田间大规模筛选的转基因植株鉴定方法.选取具有草铵膦除草剂BASTA抗性的转基因小麦进行最适BASTA溶液浓度筛选,发现在大田环境下200 mg·L-1的BASTA溶液可分别在苗期和扬花期有效鉴定转基因阳性植株.同时,为了验证BASTA叶片涂抹法的准确性和实用性,选取20个T0代转基因小麦样本,分别采用Bar试纸条、特异性PCR和BASTA叶片涂抹法3种方法进行转基因阳性鉴定.结果显示,BASTA叶片涂抹法与Bar试纸条鉴定结果一致,并且其检测范围能够覆盖特异性PCR的检测结果.与传统方法相比,BASTA叶片涂抹法成本低、高效、操作便捷,且全生育期可用,尤其适用于田间转基因植株的大规模筛选.
Abstract
Given the time-consuming and technically demanding nature of commonly used methods such as specific PCR and strip tests for detecting transgenic plants,we hoped to explore a simplified and efficient method for identifying transgenic wheat throughout its entire growth cycle in field conditions.We optimized a leaf painting method using BASTA(glufosinate herbicide),which is low-cost,straightforward,and suitable for large-scale screening of transgenic plants in wheat fields.Selecting transgenic wheat with BASTA resistance,we determined that a 200 mg·L-1 BASTA solution effectively identified transgenic positive plants during both seedling and flowering stages in field environments.Additionally,we compared this method with Bar strip tests and specific PCR on 20 T0 generation transgenic wheat plants.Results indicated that the BASTA leaf painting method correlated with the Bar strip test and covered the findings of specific PCR.Compared to traditional methods,the BASTA leaf painting method is cost-effective,efficient,operationally simple,and applicable throughout the entire growth cycle,making it particularly suitable for large-scale field screening of transgenic plants.
关键词
转基因植株/阳性鉴定/BASTA叶片涂抹法/低成本/操作简便Key words
transgenic plants/positive identification/BASTA leaf painting method/low cost/easy-to-operate引用本文复制引用
出版年
2024
NETL
NSTL
万方数据