A Low-cost,Efficient and Easy-to-operate Method for Transgenic Plant Identification
Given the time-consuming and technically demanding nature of commonly used methods such as specific PCR and strip tests for detecting transgenic plants,we hoped to explore a simplified and efficient method for identifying transgenic wheat throughout its entire growth cycle in field conditions.We optimized a leaf painting method using BASTA(glufosinate herbicide),which is low-cost,straightforward,and suitable for large-scale screening of transgenic plants in wheat fields.Selecting transgenic wheat with BASTA resistance,we determined that a 200 mg·L-1 BASTA solution effectively identified transgenic positive plants during both seedling and flowering stages in field environments.Additionally,we compared this method with Bar strip tests and specific PCR on 20 T0 generation transgenic wheat plants.Results indicated that the BASTA leaf painting method correlated with the Bar strip test and covered the findings of specific PCR.Compared to traditional methods,the BASTA leaf painting method is cost-effective,efficient,operationally simple,and applicable throughout the entire growth cycle,making it particularly suitable for large-scale field screening of transgenic plants.
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