Biofabrication of nanocomposite-based scaffolds containing human bone extracellular matrix for the differentiation of skeletal stem and progenitor cells

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外文摘要：Autograft or metal implants are routinely used in skeletal repair.However,they fail to provide long-term clinical resolution,necessitating a functional biomimetic tissue engineering alternative.The use of native human bone tissue for synthesizing a biomimetic material ink for three-dimensional(3D)bioprinting of skeletal tissue is an attractive strategy for tissue regeneration.Thus,human bone extracellular matrix(bone-ECM)offers an exciting potential for the development of an appropriate microenvironment for human bone marrow stromal cells(HBMSCs)to proliferate and differentiate along the osteogenic lineage.In this study,we engineered a novel material ink(LAB)by blending human bone-ECM(B)with nanoclay(L,Laponite®)and alginate(A)polymers using extrusion-based deposition.The inclusion of the nanofiller and polymeric material increased the rheology,printability,and drug retention properties and,critically,the preservation of HBMSCs viability upon printing.The composite of human bone-ECM-based 3D constructs containing vascular endothelial growth factor(VEGF)enhanced vascularization after implantation in an ex vivo chick chorioallantoic membrane(CAM)model.The inclusion of bone morphogenetic protein-2(BMP-2)with the HBMSCs further enhanced vascularization and mineralization after only seven days.This study demonstrates the synergistic combination of nanoclay with biomimetic materials(alginate and bone-ECM)to support the formation of osteogenic tissue both in vitro and ex vivo and offers a promising novel 3D bioprinting approach to personalized skeletal tissue repair.

外文关键词：

Extracellular matrixNanoclayBone3D bioprinting

作者：

Yang-Hee Kim、Janos M.Kanczler、Stuart Lanham、Andrew Rawlings、Marta Roldo、Gianluca Tozzi、Jonathan I.Dawson、Gianluca Cidonio、Richard O.C.Oreffo

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作者单位：

Faculty of Medicine,Bone and Joint Research Group,Centre for Human Development,Stem Cells and Regeneration,Institute of Developmental Sciences,University of Southampton,Southampton SO16 6YD,UK

School of Pharmacy and Biomedical Science,University of Portsmouth,Portsmouth PO1 2DT,UK

School of Engineering,Faculty of Engineering and Science,University of Greenwich,Greenwich ME4 4TB,UK

Center for Life Nano-and Neuro-Science(CLN2S),Italian Institute of Technology,00161 Rome,Italy

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基金：

Biotechnology and Biological Sciences Research CouncilBiotechnology and Biological Sciences Research CouncilUK Regenerative Medicine Platform Hub Acellular Approaches for Therapeutic DeliveryAcellular Hub,SMART Materials 3D ArchitectureUK Regenerative Medicine PlatformAIRC Aldi Fellowship

项目编号：

BBSRC LO21071/BB/L00609X/1MR/K026682/1MR/R015651/1MR/V00543X/125412

出版年：

2024

DOI：

10.1007/s42242-023-00265-z

生物设计与制造(英文)

ISSN：2096-5524

年,卷(期)：2024.7(2)

参考文献量36