Dissolvable temporary barrier:a novel paradigm for flexible hydrogel patterning in organ-on-a-chip models

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外文摘要：A combination of hydrogels and microfluidics allows the construction of biomimetic three-dimensional(3D)tissue models in vitro,which are also known as organ-on-a-chip models.The hydrogel patterning with a well-controlled spatial distribution is typically achieved by embedding sophisticated microstructures to act as a boundary.However,these physical barriers inevitably expose cells/tissues to a less physiologically relevant microenvironment than in vivo conditions.Herein,we present a novel dissolvable temporary barrier(DTB)strategy that allows robust and flexible hydrogel patterning with great freedom of design and desirable flow stimuli for cellular hydrogels.The key aspect of this approach is the patterning of a water-soluble rigid barrier as a guiding path for the hydrogel using stencil printing technology,followed by a barrier-free medium perfusion after the dissolution of the DTB.Single and multiple tissue compartments with different geometries can be established using either straight or curved DTB structures.The effectiveness of this strategy is further validated by generating a 3D vascular network through vasculogenesis and angiogenesis using a vascularized microtumor model.As a new proof-of-concept in vasculature-on-a-chip,DTB enables seamless contact between the hydrogel and the culture medium in closed microdevices,which is an improved protocol for the fabrication of multiorgan chips.Therefore,we expect it to serve as a promising paradigm for organ-on-a-chip devices for the development of tumor vascularization and drug evaluation in the future preclinical studies.

外文关键词：

Dissolvable temporary barrierHydrogel patterningMicrofluidicsOrgan-on-a-chipVascularization

作者：

Ding Wang、Qinyu Li、Chenyang Zhou、Zhangjie Li、Kangyi Lu、Yijun Liu、Lian Xuan、Xiaolin Wang

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作者单位：

Department of Micro/Nano Electronics,School of Electronic Information and Electrical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China

Department of Ophthalmology,LKS Faculty of Medicine,The University of Hong Kong,Hong Kong 999077,China

Institute of Medical Robotics,Shanghai Jiao Tong University,Shanghai 200240,China

National Key Laboratory of Advanced Micro and Nano Manufacture Technology,Shanghai Jiao Tong University,Shanghai 200240,China

National Center for Translational Medicine(Shanghai)SHU Branch,Shanghai University,Shanghai 200444,China

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基金：

国家自然科学基金国家自然科学基金Research Program of Shanghai Science and Technology CommitteeResearch Program of Shanghai Science and Technology Committee重庆市自然科学基金Interdisciplinary Program of Shanghai Jiao Tong UniversityInterdisciplinary Program of Shanghai Jiao Tong UniversityFoundation of National Center for Translational Medicine(Shanghai)SHU BranchCrossdisciplinary Research Fund of Shanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineCenter for Advanced Electronic Materials and Devices(AEMD)of Shanghai Jiao Tong University

项目编号：

31972929622310252114090130020DZ2220400CSTB2022NSCQ-MSX0767YG2021ZD22YG2023LC04SUITM-2023008JYJC202108

出版年：

2024

DOI：

10.1007/s42242-023-00267-x

生物设计与制造(英文)

ISSN：2096-5524

年,卷(期)：2024.7(2)

参考文献量34