首页|Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution

Highly sensitive ratiometric fluorescent fiber matrices for oxygen sensing with micrometer spatial resolution

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Oxygen(O2)-sensing matrices are promising tools for the live monitoring of extracellular O2 consumption levels in long-term cell cultures.In this study,ratiometric O2-sensing membranes were prepared by electrospinning,an easy,low-cost,scalable,and robust method for fabricating nanofibers.Poly(ε-caprolactone)and poly(dimethyl)siloxane polymers were blended with tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(Ⅱ)dichloride,which was used as the O2-sensing probe,and rhodamine B isothiocyanate,which was used as the reference dye.The functionalized scaffolds were morphologically characterized by scanning electron microscopy,and their physicochemical profiles were obtained by Fourier transform infrared spectroscopy,thermogravimetric analysis,and water contact angle measurement.The sensing capabilities were investigated by confocal laser scanning microscopy,performing photobleaching,reversibility,and calibration curve studies toward different dissolved O2(DO)concentrations.Electrospun sensing nanofibers showed a high response to changes in DO concentrations in the physiological-pathological range from 0.5％to 20％and good stability under ratiometric imaging.In addition,the sensing systems were highly biocompatible for cell growth promoting adhesiveness and growth of three cancer cell lines,namely metastatic melanoma cell line SK-MEL2,breast cancer cell line MCF-7,and pancreatic ductal adenocarcinoma cell line Panc-1,thus recreating a suitable biological environment in vitro.These O2-sensing biomaterials can potentially measure alterations in cell metabolism caused by changes in ambient O2 content during drug testing/validation and tissue regeneration processes.

ElectrospinningRuthenium(Ⅱ)dichlorideOxygen sensorsRatiometric imagingFluorescence

Giuliana Grasso、Valentina Onesto、Stefania Forciniti、Eliana D'Amone、Francesco Colella、Lara Pierantoni、Valeria Famà、Giuseppe Gigli、Rui L.Reis、J.Miguel Oliveira、Loretta L.del Mercato

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Institute of Nanotechnology,National Research Council(CNR-NANOTEC),c/o Campus Ecotekne,via Monteroni,73100 Lecce,Italy

Department of Mathematics and Physics"Ennio De Giorgi",University of Salento,c/o Campus Ecotekne,via Monteroni,73100 Lecce,Italy

3B's Research Group,I3Bs-Research Institute on Biomaterials,Biodegradables and Biomimetics,Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine,University of Minho,AvePark,Zona Industrial da Gandra,4805-017 Barco,Guimarães,Portugal

ICVS/3B's-PT Government Associate Laboratory,4806-909 Braga/Guimarães,Portugal

Department of Experimental Medicine,University of Salento,c/o Campus Ecotekne,via Monteroni,73100 Lecce,Italy

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European Research Council(ERC)under the European Union's(EU's)Horizon research and innovation program ERC Starting Grant"INTEU's Horizon research and innovation program(2020)Associazione Italiana per la Ricerca contro il Cancrothe"Tecnopolo per la medicina di precisione"(TecnoMed Puglia)—Regione Pugliathe"Tecnopolo per la medicina di precisione"(TecnoMed Puglia)—Regione PugliaItalian Ministry of Research(MUR)in the framework of the National Recovery and Resilience Plan(NRRP)NFFA-DII-PHOQScomplementary actions to the NRRP,"Fit4MedRob"Grantcomplementary actions to the NRRP,"Fit4MedRob"GrantANTHEMANTHEMNextGenerationEUPRIN funded by European Union—Next Generation EU(2022)&&Norte Portugal Regional Coordination and Development Commission(CCDR-N)under the NORTE Program(2020)AIRC Shortterm Fellowship programOpen access funding provided by Consiglio Nazionale Delle Ricerche(CNR)within the CRUI-CARE Agreement

759959953121FLAMIN-GOAIRCMFAG-2019 22902DGR n.2117 of 21/11/2018B84I18000540002n.B53C22004310006n.B53C22001750006PNC0000007n.B53C22006960001PNC0000003n.B53C220067100012022CRFNCP_PE11_PRIN2022NORTE-01-0145-FEDER-000039

2024

10.1007/s42242-024-00277-3

生物设计与制造(英文)

生物设计与制造(英文)

ISSN：2096-5524
年,卷(期)：2024.7(3)