首页|Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

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Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor progression.However,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells.This study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I collagen.It also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and migration.Finally,it compares two-dimensional(2D)and 3D cultures.For comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D model.Besides,the two models yielded significantly different cell morphologies.Finally,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D model.These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture systems.The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

HypoxiaGliomaThree-dimensional glioma modelIn vitro

Sen Wang、Siqi Yao、Na Pei、Luge Bai、Zhiyan Hao、Dichen Li、Jiankang He、J.Miguel Oliveira、Xiaoyan Xue、Ling Wang、Xinggang Mao

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State Key Laboratory for Manufacturing System Engineering,Xi'an Jiaotong University,Xi'an 710054,China

School of Mechanical Engineering,Xi'an Jiaotong University,Xi'an 710054,China

NMPA Key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices,Xi'an Jiaotong University,Xi'an 710054,China

3B's Research Group,I3Bs-Research Institute on Biomaterials,Biodegradables and Biomimetics,University of Minho,Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine,AvePark,Zona Industrial da Gandra,4805-017 Barco,Guimarães,Portugal

ICVS/3B's-PT Government Associate Laboratory,4806-909 Braga/Guimarães,Portugal

Department of Pharmacology,School of Pharmacy,Fourth Military Medical University,Xi'an,710032,China

Department of Neurosurgery,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China

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国家自然科学基金中央高校基本科研业务费专项Program for Innovation Team of Shaanxi Province,ChinaAnimal Management Committee of Xi'an Jiaotong University

522752912023-CX-TD-17XJTUAE2024-1811

2024

10.1007/s42242-024-00271-9

生物设计与制造(英文)

生物设计与制造(英文)

ISSN：2096-5524
年,卷(期)：2024.7(3)