首页|Temporal and spatial regulation of biomimetic vascularization in 3D-printed skeletal muscles

Temporal and spatial regulation of biomimetic vascularization in 3D-printed skeletal muscles

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In the intricate skeletal muscle tissue,the symbiotic relationship between myotubes and their supporting vasculature is pivotal in delivering essential oxygen and nutrients.This study explored the complex interplay between skeletal muscle and endothelial cells in the vascularization of muscle tissue.By harnessing the capabilities of three-dimensional(3D)bioprinting and modeling,we developed a novel approach involving the co-construction of endothelial and muscle cells,followed by their subsequent differentiation.Our findings highlight the importance of the interaction dynamics between these two cell types.Notably,introducing endothelial cells during the advanced phases of muscle differentiation enhanced myotube assembly.Moreover,it stimulated the development of the vascular network,paving the way for the early stages of vascularized skeletal muscle development.The methodology proposed in this study indicates the potential for constructing large-scale,physiologically aligned skeletal muscle.Additionally,it highlights the need for exploring the delicate equilibrium and mutual interactions between muscle and endothelial cells.Based on the multicell-type interaction model,we can predict promising pathways for constructing even more intricate tissues or organs.

Skeletal muscleVascularization3D bioprintingCell interaction

Minxuan Jia、Tingting Fan、Tan Jia、Xin Liu、Heng Liu、Qi Gu

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Key Laboratory of Organ Regeneration and Reconstruction,State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China

Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China

University of Chinese Academy of Sciences,Beijing 100049,China

State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China

Department of Orthopaedics,Beijing Jishuitan Hospital,Capital Medical University,Beijing 100035,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaStrategic Priority Research Program of the Chinese Academy of Sciences(CAS)CAS Project for Young Scientists in Basic Research

T2222029U21A2039662127811XDA16020802YSBR-012

2024

10.1007/s42242-024-00315-0

生物设计与制造(英文)

生物设计与制造(英文)

ISSN：2096-5524
年,卷(期)：2024.7(5)