Optimization Culture Medium Composition and Fermentation Control of Bilirubin Oxidase Producing from Bipolaris Australiensis HD-1 by a Series of Statistics Methods
In order to obtained more production of extracellar secretion of microorganism,a series of statistics methods containing 2-level factorial design,steepest ascent design and BoxBenken design of response surface method respectively were applied with optimization medium composition and fermentation control of bilirubin oxidase from Bipolaris australiensis HD-1,of which results shown that medium composition containing(g/L):maltose 5,CuSO45H2O 0.32 and soy peptone 50 at temperature of 29.8℃,pH6.8,aerobics stir 150 rpm for 6 d the enzyme activity be up to 2.18 U/mL,of which about threefold more than one of non-optimization.
BODTest design & statisticsOptimization medium composition and fermentation control
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