An Optimization Algorithm for the Kinetics Mechanism Discrimination of The Double Substrates Enzymatic Reaction
A common used method to research the kinetics mechanism of double substrates enzymatic reaction is double-reciprocal plots.In this paper,an optimization algorithm based on the last squares fitting principle is proposed to solve the disadvantages in double-reciprocal plots method.In the optimization algorithm,the constant coefficients of the double-reciprocal kinetics equations for both sequential mechanism and ping-pong mechanism are calculated by the last squares fitting.The kinetics mechanism of the enzymatic reaction is distinguished through the comparison of residual sum of squares for the two curve-fitting functions.An example is specified to prove that the optimization algorithm is concise and reliable,and is more accurate in the double substrates enzymatic reaction kinetics mechanism discrimination.
NETL
NSTL
万方数据
维普
