Amplification of DNA fragments and electrophoretic identification:Improvement of the experiment
In teaching"basic operational procedures of genetic engineering",the experiment of amplification of DNA fragments and electrophoretic identification can stimulate students'interest in science,train their scientific inquiry ability,and thereby enhance students'core literacy for biology.Traditional experimental teaching often involves long experiment durations and safety issues related to procedures such as"sample loading".By applying the latest experimental instruments and improving operational steps,experiments can be completed within a single class,thereby increasing efficiency.Improvements in experimental materials ensure safety,and the innovative use of anthocyanin fluorescent dyes presents more desirable visualization,making it worthy of promotion and application.
PCRelectrophoresisimprovement of the experimentsenior high school biology