To clone chalcone synthase ( CHS) gene from the Citrus grandis tomentosa, which was the genuine medicinal materials from Guangdong province, CTAB-LiCl treatment was used to extract RNA from Citrus grandis tomentosa' and RT-PCR was used to clone the CHS gene at the same time. The CHS gene included the open reading frame( ORF) , and it was consisted of 1173bp nucleic acid, encoded 391 amino acid residues. Compared with other CHS genes from citrus plant, it was found that they shared a sequence homology more than 98％ at the nucleic acid level. Result showed that CTAB-LiCl treatment could extract high quality RNA from Citrus grandis tomentosa'. The cloned CHS gene from Citrus grandis tomentosa' had the open reading frame and shared a high sequence homology with other Citrus plant.
NETL
NSTL
维普
万方数据
