The structure and organization of mitochondrial genomes of six species of Epinephelus and one Plectropomus leopardus samples were analyzed using bioinformatics method.The basic characteristics including protein coding genes,different loci,selection pressure and phylogenetic information were studied in detail.The results showed that mitochondrial genome composition and gene order was consistent with other teleost fishes.There are 37 standard genes; among all the genes,the ND6 had the largest (33.9%) and 16SrRNA had the smallest (13.5%) genetic differences proportion.13 protein coding genes were mainly acted by purifying selection as dctected by Tajima' s D test,and all of them were influenced by purifying selection (Ka/Ks < 1).Combined with topological structure and bootstrap value sum of phylogenetic tree.Results showed Cytb and ND2 genes as suitable molecular markers for phylogenetic study of Epinephelus fishes.