Lactobacillus plantarum is one kind of lactobacillus often found in fermented vegtable and fruits .It can maintain the balance of bacteria in gastrointestinal tract , enhance body′s immune system and promote nutrient absorption .The L.plantarum was obtained from commercial pickles .The oxford cup agar well diffusion method was used to detect the diameter of bacteriostatic circle , and the re-sults showed that and L.plantarum could produce broad-spectrum antimicrobial substance , which had strongly restraining effect on Escherichia coli, Staphylococcus citreus, Micrococcus luteus and Bacillus subtilis.The biological characteristics of L.plantarum included the effects of heat, pH, enzymes.The results indicated that it had good heat stability and remained activity after incubation at a wide pH.It was sensitive to some proteases , such as proteinase K , pepsinum and trypsinase .This study would make a foundation for further developing the plant lactobacillus .