Research on rapid propagation technology of tissue culture of Cymbidium
The 1053 freshmen Cymbidium seedling variety were chosen as explants , using MS as basic medium for tissue culture of Cymbidium.Experiment results show that PLB -induced is optimal explant for the Cymbidium bulbs , the optimal ratio of hormone con-centrations is 0.5mg/L 6-BA +0.2mg/L NAA.PLB optimal hormone concentration ratio of proliferation of protocorm and differentia-tion of bud is 1.0mg/L 6-BA +0.3mg/L NAA;the bud growth hormone concentration ratio is 2.0mg/L6-BA +0.5mg/LNAA;or-ganic additive is 150ml /L coconut.The bud proliferation orthogonal test results show that primary and secondary order are 6-BA >coconut additives >NAA, suitable combination is 2mg/L 6-BA, 0.5mg/L NAA, coconut milk additives 150ml /L.The rooting hor-mone concentration ratio is 0.3mg/L IBA +0.2mg/L NAA, adding 100g/L banana mud has significant effect on the growing of the seedling root .
CymbidiumPLB inductionbud proliferationorthogonal test
NETL
NSTL
万方数据
维普
