摘要
为深入探讨蔗糖在植物花青素生物合成过程中的调节作用，以紫色土豆为材料，分析不同蔗糖浓度对紫色土豆外植体生长、花青素含量、微型薯形成以及花青素生物合成相关基因表达的影响。结果表明，紫色土豆外植体在蔗糖浓度为15 g／L时生长情况最好，平均长度最长，平均重量最重，但高浓度蔗糖对外植体生长起抑制作用；蔗糖能促进紫色土豆花青素含量的积累及微型薯的形成，随着蔗糖浓度的增加，花青素含量随之增加，紫色土豆花青素含量在蔗糖浓度为120 g／L时最高，为5.11 mg／g FW，是对照组的8.89倍；蔗糖浓度为60 g／L时，微型薯重量最重，为0.34 g，结实率最高，达到22.50％。 RT-PCR结果显示，蔗糖能促进StF3′5′H、StUFGT以及StDFR的表达，蔗糖通过调控此类花青素合成相关基因的表达影响紫色土豆花青素含量。
Abstract
To illuminate the sucrose regulation of plant anthocyanin biosynthesis pathway , the effects of different sucrose concentration on the explants growth , anthocyanin accumulation , the minituber formation and the related gene expression involved in anthocyanin bio -synthesis in purple potato were investigated .The results indicated that when cultured with 15 g/L sucrose , the explants have the best growth condition , the growth of explants was inhibited at high concentration of sucrose .Sucrose enhanced the contents of anthocyanin and the minituber formation in purple potato .With the increasing of sucrose concentration , anthocyanin accumulation was significantly increased.When treated with 120 g/L sucrose, the contents of anthocyanin achieved the highest value as 5.11 mg/g FW.The minitu-bers have a heaviest weight (0.34 g) and highest setting rate (22.50%), when treated with 60 g/L sucrose.The related genes in-volved in anthocyanin biosynthesis including StF3′5′H, StDFR and StUFGT were up-regulated by sucrose , implying that the sucrose might control the anthocyanin accumulation by regulating the genes expressions involved in anthocyanin biosynthesis .
NETL
NSTL
维普
万方数据