In order to investigate the difference in morphology of cerebellar Purkinje cells in mice at different ages,mice were divided into four age groups of five animals each corresponding to 14-day,1-month,2-month,and 1-year old mice.Parvalbumin selected as a unique marker for Purkinje cells,using immunochemistry,we observed and compared somatic and dendritic morphology of cerebellar Purkinje cells of these mice in four groups under a confocal microscope.The results showed that the dendritic length of cerebellar Purkinje cells in 2-month old mice was significantly higher than that in other groups(P＜0.05),and the dendritic length of cerebellar Purkinje cells in 1-year old mice was significantly lower than that in other groups(P＜0.05),as well as the somatic sizes of Purkinje cells of mice in 4 age groups showed a decreasing trend with age.In this study,we also analyzed the fluorescence intensity of parvalbu-min in cerebellar Purkinje cells of mice in 4 age groups,which showed an overall increasing trend with age.Together,the results indi-cate that cell body size and dendritic length of cerebellar Purkinje are associated with age,with an opposite trend of parvalbumin ex-pression and its development.This study suggests that parvalbumin may play an important role in regulation of cerebellar Purkinje cell development.
维普
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