This study investigated the protective effects and mechanisms of lavender essential oil(LEO)on ultraviolet light induced damage of skin barrier.The components of LEO were identified and analyzed by gas chromatography-mass spectrometry(GC-MS).3D epidermal skin models were used to establish skin barrier damaged model by ultraviolet B(UVB)irritation at a dose of 600 mJ/cm2.The non-medication without UVB exposure group,non-medication with UVB exposure group,vitamin E/dexamethasone treatment with UVB exposure group and LEO treatment with UVB exposure group were set up parallelly.The effects of different treatment on oxidative stress,inflammation,barrier structure-related proteins and CD44 receptor were investigated by hematoxylin-eosin staining,enzyme-linked immunosorbent assay,immunohistochemistry and immunofluorescence methods,etc.The results showed that a total of 51 chem-ical components of LEO were identified by GC-MS,and the main components were linalyl acetate,linalool,lavandulyl acetate,etc.UVB could cause damage to skin barrier exhibiting increasing ROS level and expression of inflammatory factors and decreasing SOD en-zyme activity and expression of barrier-related proteins and CD44 receptors.LEO had a certain protective effect on UVB-induced skin barrier damage mainly by inhibiting UVB-induced increase in the expressions of IL-1α,TNF-α and PGE2 and ROS production(P＜0.05)and by increasing the expression of four barrier-related proteins and CD44 receptor,and SOD enzyme activity(P＜0.01)in the skin barrier damaged models.These results indicated that LEO had a great application prospect in reducing UVB damage of skin barri-er,which provided basis for its application in skin care products development.
维普
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