The mitochondrial genome and phylogenetic analysis of Narosa fulgens(Leech,1889)
To explore the mitochondrial genome of Narosa fulgens(Leech,1889)and the phylogeny of Limacodidae,we newly se-quenced the complete mitochondrial genome of N.fulgens(Lepidoptera,Limacodidae),by using the next-generation sequencing meth-od.In phylogenetic analyses,we selected 16 exemplars of Zygaenoidea as ingroups.Each onespecies of Cossidae and Thyrididae was used as outgroup.We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods.The se-quenced genome is a circular molecule of 15 296 bp in length(GenBank accession No.OP919326)which possesses 13 protein-coding genes,22 transfer RNA genes,2 ribosomal RNA genes,and putative control regions as well.For most of the protein-coding genes,the star codons were ATN except for cox1 using CGA as star codon.Most of the protein-coding genes terminated with the stop codon TAA,while cox2 and nad4 employed the incomplete T as the stop codon.All tRNA genes can be folded into atypical cloverleaf structure,with the except of the trnS1-AGN.The trnS1 lacks the DHU arm.The lengths of the rrnL and rrnS genes were 1 335 bp and 732 bp,respectively,with the A+T contents of 85.24％and 84.70％.Both phylogenetic inference methods showed largely identical tree topological structures:the families Limacodidae and Zygaenidae were respectively monophyletic,Narosa fulgens is sister to Narosa nigrisigna.
万方数据
维普
